Uncharacterized protein
AF-A0A2V0NZB6-F1-v4
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Predicted aligned error (PAE)
Click and drag a box on the PAE viewer to select regions of the structure and highlight them on the 3D viewer.
PAE data is useful for assessing inter-domain accuracy – go to Help section below for more information.
Start a structural similarity search to discover similar proteins.
AlphaFold database protein sequences clustered by the MMseqs2 algorithm (Steinegger M. and Soeding J., Nat. Commun. 9, 2018). Each cluster is comprised of sequences that fulfil two criteria: maintaining a maximum sequence identity of 50% and achieving a 90% bi-directional sequence overlap with the longest sequence of the cluster representative.
AFDB accession | Description | Species | Sequence length | Average pLDDT |
---|---|---|---|---|
AFDB accessionAF-A0A1B8E8M1-F1 | Description Uncharacterized protein Uncharacterized protein | SpeciesPseudogymnoascus sp. 23342-1-I1 Pseudogymnoascus sp. 23342-1-I1 | Sequence length 668 | Average pLDDT 91.62 |
AFDB accessionAF-A0A162IUL2-F1 | Description Periodic tryptophan protein 2 Periodic tryptophan protein 2 | SpeciesMoelleriella libera RCEF 2490 Moelleriella libera RCEF 2490 | Sequence length 852 | Average pLDDT 91.31 |
AFDB accessionAF-A0A6A7BTM5-F1 | Description WD40 repeat-like protein WD40 repeat-like protein | SpeciesPiedraia hortae CBS 480.64 Piedraia hortae CBS 480.64 | Sequence length 846 | Average pLDDT 91.19 |
AFDB accessionAF-A0A2T0FF52-F1 | Description Periodic tryptophan protein 2 Periodic tryptophan protein 2 | SpeciesWickerhamiella sorbophila Wickerhamiella sorbophila | Sequence length 835 | Average pLDDT 91 |
AFDB accessionAF-A0A381L8S7-F1 | Description Bgt-1678 Bgt-1678 | SpeciesBlumeria graminis f. sp. tritici 96224 Blumeria graminis f. sp. tritici 96224... Blumeria graminis f. sp. tritici 96224 | Sequence length 864 | Average pLDDT 90.81 |
AFDB accessionAF-A0A1E3Q4Q6-F1 | Description Utp12 domain-containing protein Utp12 domain-containing protein | SpeciesLipomyces starkeyi NRRL Y-11557 Lipomyces starkeyi NRRL Y-11557 | Sequence length 856 | Average pLDDT 90.81 |
AFDB accessionAF-A0A2N1LI23-F1 | Description Utp12 domain-containing protein Utp12 domain-containing protein | SpeciesTrichoderma harzianum Trichoderma harzianum | Sequence length 876 | Average pLDDT 90.75 |
AFDB accessionAF-N1JJH8-F1 | Description Periodic tryptophan protein-like protein Periodic tryptophan protein-like protein ... Periodic tryptophan protein-like protein | SpeciesBlumeria graminis f. sp. hordei (strain DH14) Blumeria graminis f. sp. hordei (strain DH14)... Blumeria graminis f. sp. hordei (strain DH14) | Sequence length 864 | Average pLDDT 90.62 |
AFDB accessionAF-S9W6U9-F1 | Description U3 snoRNP-associated protein Utp1 U3 snoRNP-associated protein Utp1 | SpeciesSchizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691) Schizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691)... Schizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691) | Sequence length 854 | Average pLDDT 90.56 |
AFDB accessionAF-A0A5J5ERQ6-F1 | Description WD40-repeat-containing domain protein WD40-repeat-containing domain protein ... WD40-repeat-containing domain protein | SpeciesSphaerosporella brunnea Sphaerosporella brunnea | Sequence length 861 | Average pLDDT 90.38 |
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How to interpret the Predicted Aligned Error
The Predicted Aligned Error (PAE) measures the confidence in the relative position of two residues within the predicted structure, providing insight into the reliability of relative position and orientations of different domains. Consider the human protein encoded by the gene GNE (Q9Y223). GNE has two distinct domains according to experimentally determined structures in the Protein Data Bank (PDBe-KB). Does AlphaFold confidently predict their relative positions? We can use the interactive Predicted Aligned Error (PAE) plot to answer this question. The PAE plot is not an inter-residue distance map or a contact map. Instead, the shade of green indicates the expected distance error in Ångströms (Å), ranging from 0 Å to an arbitrary cut-off of 31 Å. The colour at (x, y) corresponds to the expected distance error in the residue x’s position when the predicted and the true structures are aligned on residue y. The two low-error, dark green squares correspond to the two domains. By clicking and dragging, you can highlight these squares on the structure. If you want to remove the highlighting, click the cross icon. When selecting an off-diagonal region, the plot visually represents the relationship between the selected ranges on the sequence and structure. The x range corresponds to the selection for scored residues, highlighted in orange, while the y range of aligned residues is highlighted in emerald green. Let’s consider another inter-domain example, the human protein encoded by DIP2B (Q9P265). In this case, we have confidence in the relative position of scored residues around 1450 when aligned with residues around 850, suggesting a packing between the small central domains. Note that the PAE scores are asymmetrical, meaning there might be variations in PAE values between (x,y) and (y,x) positions. This is particularly relevant for loop regions with highly uncertain orientations, as seen on the DNA topoisomerase 3 (Q8T2T7).
A dark green tile corresponds to a good prediction (low error), whereas a light green tile indicates poor prediction (high error). For example, when aligning on residue 300:
The high PAE values across the whole inter-domain region indicate that for this particular protein, AlphaFold does not reliably predict the relative position of the domains.
Last updated
Last updated in AlphaFold DB version 2022-11-01, created with the AlphaFold Monomer v2.0 pipeline.
Licence and attribution
Data is available for academic and commercial use, under a CC-BY-4.0 licence.
EMBL-EBI expects attribution (e.g. in publications, services or products) for any of its online services, databases or software in accordance with good scientific practice.
If you make use of an AlphaFold prediction, please cite the following papers: Jumper, J et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021).
Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024).
If you use data from AlphaMissense in your work, please cite the following paper: Cheng, J et al. Accurate proteome-wide missense variant effect prediction with AlphaMissense. Science (2023).
AlphaFold Data Copyright (2022) DeepMind Technologies Limited.
AlphaMissense Copyright (2023) DeepMind Technologies Limited.
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Disclaimer
The AlphaFold and AlphaMissense Data and other information provided on this site contain predictions with varying levels of confidence, is for theoretical modelling only and caution should be exercised in its use. It is provided 'as-is' without any warranty of any kind, whether expressed or implied. For clarity, no warranty is given that use of the information shall not infringe the rights of any third party. The information is not intended to be a substitute for professional medical advice, diagnosis, or treatment, and does not constitute medical or other professional advice. The AlphaFold and AlphaMissense Data have not been validated for, and are not approved for, any clinical use.
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