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MFS domain-containing protein

Unreviewed
Reference proteome

AF-A0A7M7GPD4-F1-v4

DownloadPDB file mmCIF file Predicted aligned error

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Information

Structure viewer
Model Confidence
pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test (lDDT-Cα). It is a measure of local accuracy - for interpreting larger scale features like relative domain positions see the “predicted aligned error” plot and corresponding tutorial. Confidence bands are used to colour-code the residues in the 3D viewer. The exact pLDDT value is shown when you mouseover the structure or the sequence. It can also be found in the B-factor fields of the downloadable coordinate files.

 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.

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SequenceNo structure available
Structure Tools
  • 6:57:54
    Mol* Plugin 4.5.0 [8/22/2024, 2:35:37 PM]
Model Confidence
pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test (lDDT-Cα). It is a measure of local accuracy - for interpreting larger scale features like relative domain positions see the “predicted aligned error” plot and corresponding tutorial. Confidence bands are used to colour-code the residues in the 3D viewer. The exact pLDDT value is shown when you mouseover the structure or the sequence. It can also be found in the B-factor fields of the downloadable coordinate files.
 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.
Scored residueAligned residue
01002003004005000100200300400500
  • 0
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
Expected position error (Ångströms)

Predicted aligned error (PAE)

Click and drag a box on the PAE viewer to select regions of the structure and highlight them on the 3D viewer.

PAE data is useful for assessing inter-domain accuracy – go to Help section below for more information.

Similar structures Discover similar structures from the Protein Data Bank (PDB) and the AlphaFold Database clustered to 50% sequence identity (AFDB50) using Foldseek.
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Structure similarity clusterPredicted structures in the AlphaFold Protein Structure Database clustered using MMseqs2 and Foldseek. This data is provided by the AFDB Clusters.

AlphaFold database protein sequences clustered by the MMseqs2 algorithm (Steinegger M. and Soeding J., Nat. Commun. 9, 2018). Each cluster is comprised of sequences that fulfil two criteria: maintaining a maximum sequence identity of 50% and achieving a 90% bi-directional sequence overlap with the longest sequence of the cluster representative.

AFDB accession DescriptionSpecies
Sequence length
Average pLDDT
AFDB accessionAF-A0A1Y1LRF2-F1
Unreviewed
Reference proteome
Description MFS_1_like domain-containing protein

MFS_1_like domain-containing protein ...

MFS_1_like domain-containing protein
SpeciesPhotinus pyralis
Photinus pyralis
Sequence length 512 Average pLDDT 81.69
AFDB accessionAF-B7PLT8-F1
Unreviewed
Reference proteome
Description MFS_1_like domain-containing protein

MFS_1_like domain-containing protein ...

MFS_1_like domain-containing protein
SpeciesIxodes scapularis
Ixodes scapularis
Sequence length 565 Average pLDDT 80.69
AFDB accessionAF-A0A1E1XM29-F1
Unreviewed
Description Putative transporter
Putative transporter
SpeciesAmblyomma sculptum
Amblyomma sculptum
Sequence length 577 Average pLDDT 80.44
AFDB accessionAF-A0A154PKN9-F1
Unreviewed
Reference proteome
Description Major facilitator superfamily domain-containing protein 6

Major facilitator superfamily domain-containing protein 6 ...

Major facilitator superfamily domain-containing protein 6
SpeciesDufourea novaeangliae
Dufourea novaeangliae
Sequence length 532 Average pLDDT 80.12
AFDB accessionAF-A0A1Y1LZ67-F1
Unreviewed
Description MFS_1_like domain-containing protein

MFS_1_like domain-containing protein ...

MFS_1_like domain-containing protein
SpeciesPhotinus pyralis
Photinus pyralis
Sequence length 496 Average pLDDT 80.06
AFDB accessionAF-A0A6J3JVY2-F1
Unreviewed
Reference proteome
Description major facilitator superfamily domain-containing protein 6 isoform X2

major facilitator superfamily domain-containing protein 6 isoform X2 ...

major facilitator superfamily domain-containing protein 6 isoform X2
SpeciesBombus vosnesenskii
Bombus vosnesenskii
Sequence length 531 Average pLDDT 79.75
AFDB accessionAF-L7M0G8-F1
Unreviewed
Description Putative transporter
Putative transporter
SpeciesRhipicephalus pulchellus
Rhipicephalus pulchellus
Sequence length 567 Average pLDDT 79.69
AFDB accessionAF-A0A6P8MBA7-F1
Unreviewed
Reference proteome
Description major facilitator superfamily domain-containing protein 6 isoform X1

major facilitator superfamily domain-containing protein 6 isoform X1 ...

major facilitator superfamily domain-containing protein 6 isoform X1
SpeciesBombus bifarius
Bombus bifarius
Sequence length 531 Average pLDDT 79.62
AFDB accessionAF-A0A7M7GPD4-F1
Unreviewed
Reference proteome
Description MFS domain-containing protein
MFS domain-containing protein
SpeciesApis mellifera
Apis mellifera
Sequence length 530 Average pLDDT 79.62
AFDB accessionAF-A0A6P3D4E2-F1
Unreviewed
Reference proteome
Description major facilitator superfamily domain-containing protein 6

major facilitator superfamily domain-containing protein 6 ...

major facilitator superfamily domain-containing protein 6
SpeciesIxodes scapularis
Ixodes scapularis
Sequence length 565 Average pLDDT 79.56
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Help

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How to interpret the Predicted Aligned Error

 

The Predicted Aligned Error (PAE) measures the confidence in the relative position of two residues within the predicted structure, providing insight into the reliability of relative position and orientations of different domains. Consider the human protein encoded by the gene GNE (Q9Y223).

GNE has two distinct domains according to experimentally determined structures in the Protein Data Bank (PDBe-KB). Does AlphaFold confidently predict their relative positions? We can use the interactive Predicted Aligned Error (PAE) plot to answer this question.

The PAE plot is not an inter-residue distance map or a contact map. Instead, the shade of green indicates the expected distance error in Ångströms (Å), ranging from 0 Å to an arbitrary cut-off of 31 Å. The colour at (x, y) corresponds to the expected distance error in the residue x’s position when the predicted and the true structures are aligned on residue y.

A dark green tile corresponds to a good prediction (low error), whereas a light green tile indicates poor prediction (high error). For example, when aligning on residue 300:

  • We’re confident in the relative position of residue 200
  • We’re not confident in the relative position of residue 600

The two low-error, dark green squares correspond to the two domains. By clicking and dragging, you can highlight these squares on the structure. If you want to remove the highlighting, click the cross icon.

When selecting an off-diagonal region, the plot visually represents the relationship between the selected ranges on the sequence and structure. The x range corresponds to the selection for scored residues, highlighted in orange, while the y range of aligned residues is highlighted in emerald green.

The high PAE values across the whole inter-domain region indicate that for this particular protein, AlphaFold does not reliably predict the relative position of the domains.

Let’s consider another inter-domain example, the human protein encoded by DIP2B (Q9P265).

In this case, we have confidence in the relative position of scored residues around 1450 when aligned with residues around 850, suggesting a packing between the small central domains.

Note that the PAE scores are asymmetrical, meaning there might be variations in PAE values between (x,y) and (y,x) positions. This is particularly relevant for loop regions with highly uncertain orientations, as seen on the DNA topoisomerase 3 (Q8T2T7).

 


Last updated

Last updated in AlphaFold DB version 2022-11-01, created with the AlphaFold Monomer v2.0 pipeline.

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If you make use of an AlphaFold prediction, please cite the following papers:
Jumper, J et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021).
Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024).
If you use data from AlphaMissense in your work, please cite the following paper:
Cheng, J et al. Accurate proteome-wide missense variant effect prediction with AlphaMissense. Science (2023).

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AlphaMissense Copyright (2023) DeepMind Technologies Limited.

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