Transposase
AF-A0A849SYW0-F1-v4
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Predicted aligned error (PAE)
Click and drag a box on the PAE viewer to select regions of the structure and highlight them on the 3D viewer.
PAE data is useful for assessing inter-domain accuracy – go to Help section below for more information.
Start a structural similarity search to discover similar proteins.
AlphaFold database protein sequences clustered by the MMseqs2 algorithm (Steinegger M. and Soeding J., Nat. Commun. 9, 2018). Each cluster is comprised of sequences that fulfil two criteria: maintaining a maximum sequence identity of 50% and achieving a 90% bi-directional sequence overlap with the longest sequence of the cluster representative.
AFDB accession | Description | Species | Sequence length | Average pLDDT |
---|---|---|---|---|
AFDB accessionAF-A0A829NXE4-F1 | Description Transposase IS110 Transposase IS110 | SpeciesRhodobacter capsulatus YW2 Rhodobacter capsulatus YW2 | Sequence length 155 | Average pLDDT 95 |
AFDB accessionAF-Q0FHT8-F1 | Description Transposase Transposase | SpeciesSalipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601) Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601)... Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601) | Sequence length 170 | Average pLDDT 94.5 |
AFDB accessionAF-A0A529KZ11-F1 | Description Transposase Transposase | SpeciesMesorhizobium sp Mesorhizobium sp | Sequence length 124 | Average pLDDT 94.25 |
AFDB accessionAF-D0CR22-F1 | Description ISPsy16, transposase ISPsy16, transposase | SpeciesRuegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157)... Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) | Sequence length 140 | Average pLDDT 92.38 |
AFDB accessionAF-V7EQX8-F1 | Description DEDD_Tnp_IS110 domain-containing protein DEDD_Tnp_IS110 domain-containing protein ... DEDD_Tnp_IS110 domain-containing protein | SpeciesMesorhizobium sp. LSJC264A00 Mesorhizobium sp. LSJC264A00 | Sequence length 164 | Average pLDDT 91.5 |
AFDB accessionAF-A0A849SYW0-F1 | Description Transposase Transposase | SpeciesBacteriovoracaceae bacterium Bacteriovoracaceae bacterium | Sequence length 178 | Average pLDDT 91.12 |
AFDB accessionAF-A0A1V0GU01-F1 | Description IS110 family transposase IS110 family transposase | SpeciesParacoccus yeei Paracoccus yeei | Sequence length 177 | Average pLDDT 89.69 |
AFDB accessionAF-A0A2M7JYP2-F1 | Description IS110 family transposase IS110 family transposase | SpeciesChloroflexi bacterium CG_4_8_14_3_um_filter_45_15 Chloroflexi bacterium CG_4_8_14_3_um_filter_45_15... Chloroflexi bacterium CG_4_8_14_3_um_filter_45_15 | Sequence length 145 | Average pLDDT 88.5 |
AFDB accessionAF-A0A2M6YAW0-F1 | Description DEDD_Tnp_IS110 domain-containing protein DEDD_Tnp_IS110 domain-containing protein ... DEDD_Tnp_IS110 domain-containing protein | SpeciesChloroflexi bacterium CG08_land_8_20_14_0_20_45_12 Chloroflexi bacterium CG08_land_8_20_14_0_20_45_12... Chloroflexi bacterium CG08_land_8_20_14_0_20_45_12 | Sequence length 147 | Average pLDDT 87.94 |
AFDB accessionAF-Q8DQ71-F1 | Description Degenerate transposase Degenerate transposase | SpeciesStreptococcus pneumoniae (strain ATCC BAA-255 / R6) Streptococcus pneumoniae (strain ATCC BAA-255 / R6)... Streptococcus pneumoniae (strain ATCC BAA-255 / R6) | Sequence length 175 | Average pLDDT 87.4 |
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How to interpret the Predicted Aligned Error
The Predicted Aligned Error (PAE) measures the confidence in the relative position of two residues within the predicted structure, providing insight into the reliability of relative position and orientations of different domains. Consider the human protein encoded by the gene GNE (Q9Y223). GNE has two distinct domains according to experimentally determined structures in the Protein Data Bank (PDBe-KB). Does AlphaFold confidently predict their relative positions? We can use the interactive Predicted Aligned Error (PAE) plot to answer this question. The PAE plot is not an inter-residue distance map or a contact map. Instead, the shade of green indicates the expected distance error in Ångströms (Å), ranging from 0 Å to an arbitrary cut-off of 31 Å. The colour at (x, y) corresponds to the expected distance error in the residue x’s position when the predicted and the true structures are aligned on residue y. The two low-error, dark green squares correspond to the two domains. By clicking and dragging, you can highlight these squares on the structure. If you want to remove the highlighting, click the cross icon. When selecting an off-diagonal region, the plot visually represents the relationship between the selected ranges on the sequence and structure. The x range corresponds to the selection for scored residues, highlighted in orange, while the y range of aligned residues is highlighted in emerald green. Let’s consider another inter-domain example, the human protein encoded by DIP2B (Q9P265). In this case, we have confidence in the relative position of scored residues around 1450 when aligned with residues around 850, suggesting a packing between the small central domains. Note that the PAE scores are asymmetrical, meaning there might be variations in PAE values between (x,y) and (y,x) positions. This is particularly relevant for loop regions with highly uncertain orientations, as seen on the DNA topoisomerase 3 (Q8T2T7).
A dark green tile corresponds to a good prediction (low error), whereas a light green tile indicates poor prediction (high error). For example, when aligning on residue 300:
The high PAE values across the whole inter-domain region indicate that for this particular protein, AlphaFold does not reliably predict the relative position of the domains.
Last updated
Last updated in AlphaFold DB version 2022-11-01, created with the AlphaFold Monomer v2.0 pipeline.
Licence and attribution
Data is available for academic and commercial use, under a CC-BY-4.0 licence.
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If you make use of an AlphaFold prediction, please cite the following papers: Jumper, J et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021).
Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024).
If you use data from AlphaMissense in your work, please cite the following paper: Cheng, J et al. Accurate proteome-wide missense variant effect prediction with AlphaMissense. Science (2023).
AlphaFold Data Copyright (2022) DeepMind Technologies Limited.
AlphaMissense Copyright (2023) DeepMind Technologies Limited.
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