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Bifunctional protein HldE

Reviewed

AF-B5YR91-F1-v4

DownloadPDB file mmCIF file Predicted aligned error

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Structure viewer
Model Confidence
pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test (lDDT-Cα). It is a measure of local accuracy - for interpreting larger scale features like relative domain positions see the “predicted aligned error” plot and corresponding tutorial. Confidence bands are used to colour-code the residues in the 3D viewer. The exact pLDDT value is shown when you mouseover the structure or the sequence. It can also be found in the B-factor fields of the downloadable coordinate files.

 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.

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SequenceNo structure available
Structure Tools
  • 12:14:25
    Mol* Plugin 4.5.0 [8/22/2024, 2:35:37 PM]
Model Confidence
pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test (lDDT-Cα). It is a measure of local accuracy - for interpreting larger scale features like relative domain positions see the “predicted aligned error” plot and corresponding tutorial. Confidence bands are used to colour-code the residues in the 3D viewer. The exact pLDDT value is shown when you mouseover the structure or the sequence. It can also be found in the B-factor fields of the downloadable coordinate files.
 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.
Scored residueAligned residue
01002003004000100200300400
  • 0
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
Expected position error (Ångströms)

Predicted aligned error (PAE)

Click and drag a box on the PAE viewer to select regions of the structure and highlight them on the 3D viewer.

PAE data is useful for assessing inter-domain accuracy – go to Help section below for more information.

Similar structures Discover similar structures from the Protein Data Bank (PDB) and the AlphaFold Database clustered to 50% sequence identity (AFDB50) using Foldseek.
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Structure similarity clusterPredicted structures in the AlphaFold Protein Structure Database clustered using MMseqs2 and Foldseek. This data is provided by the AFDB Clusters.

AlphaFold database protein sequences clustered by the MMseqs2 algorithm (Steinegger M. and Soeding J., Nat. Commun. 9, 2018). Each cluster is comprised of sequences that fulfil two criteria: maintaining a maximum sequence identity of 50% and achieving a 90% bi-directional sequence overlap with the longest sequence of the cluster representative.

AFDB accession DescriptionSpecies
Sequence length
Average pLDDT
AFDB accessionAF-A0A7T8GDN0-F1
Unreviewed
Description 2-dehydro-3-deoxy-phosphogluconate aldolase

2-dehydro-3-deoxy-phosphogluconate aldolase ...

2-dehydro-3-deoxy-phosphogluconate aldolase
SpeciesProteus vulgaris
Proteus vulgaris
Sequence length 530 Average pLDDT 92.62
AFDB accessionAF-Q74BF6-F1
Reviewed (Swiss-Prot)
Reference proteome
Description Bifunctional protein HldE
Bifunctional protein HldE
SpeciesGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)...

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Sequence length 490 Average pLDDT 92.61
AFDB accessionAF-A0A6G6SN02-F1
Unreviewed
Reference proteome
Description 2-dehydro-3-deoxy-phosphogluconate aldolase

2-dehydro-3-deoxy-phosphogluconate aldolase ...

2-dehydro-3-deoxy-phosphogluconate aldolase
SpeciesProteus vulgaris
Proteus vulgaris
Sequence length 526 Average pLDDT 92.5
AFDB accessionAF-A0A1G0JID5-F1
Unreviewed
Reference proteome
Description Bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase ...

Bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase
SpeciesGammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_57_10

Gammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_57_10...

Gammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_57_10
Sequence length 433 Average pLDDT 92.19
AFDB accessionAF-B3E5M9-F1
Reviewed (Swiss-Prot)
Reference proteome
Description Bifunctional protein HldE
Bifunctional protein HldE
SpeciesGeobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)

Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)...

Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ)
Sequence length 491 Average pLDDT 92.17
AFDB accessionAF-Q7N0C3-F1
Reviewed (Swiss-Prot)
Reference proteome
Description Bifunctional protein HldE
Bifunctional protein HldE
SpeciesPhotorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)...

Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)
Sequence length 474 Average pLDDT 91.8
AFDB accessionAF-A6W0Z9-F1
Reviewed (Swiss-Prot)
Reference proteome
Description Bifunctional protein HldE
Bifunctional protein HldE
SpeciesMarinomonas sp. (strain MWYL1)
Marinomonas sp. (strain MWYL1)
Sequence length 475 Average pLDDT 91.71
AFDB accessionAF-A0A7T5ITU6-F1
Unreviewed
Reference proteome
Description D-glycero-beta-D-manno-heptose-7-phosphate kinase

D-glycero-beta-D-manno-heptose-7-phosphate kinase ...

D-glycero-beta-D-manno-heptose-7-phosphate kinase
SpeciesCampylobacter fetus subsp. venerealis

Campylobacter fetus subsp. venerealis...

Campylobacter fetus subsp. venerealis
Sequence length 458 Average pLDDT 91.69
AFDB accessionAF-A0A3M4EXT9-F1
Unreviewed
Reference proteome
Description D-beta-D-heptose 1-phosphate adenylyltransferase

D-beta-D-heptose 1-phosphate adenylyltransferase ...

D-beta-D-heptose 1-phosphate adenylyltransferase
SpeciesPseudomonas syringae pv. atrofaciens

Pseudomonas syringae pv. atrofaciens...

Pseudomonas syringae pv. atrofaciens
Sequence length 451 Average pLDDT 91.69
AFDB accessionAF-Q39X60-F1
Reviewed (Swiss-Prot)
Reference proteome
Description Bifunctional protein HldE
Bifunctional protein HldE
SpeciesGeobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)

Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)...

Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
Sequence length 490 Average pLDDT 91.69
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Help

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How to interpret the Predicted Aligned Error

 

The Predicted Aligned Error (PAE) measures the confidence in the relative position of two residues within the predicted structure, providing insight into the reliability of relative position and orientations of different domains. Consider the human protein encoded by the gene GNE (Q9Y223).

GNE has two distinct domains according to experimentally determined structures in the Protein Data Bank (PDBe-KB). Does AlphaFold confidently predict their relative positions? We can use the interactive Predicted Aligned Error (PAE) plot to answer this question.

The PAE plot is not an inter-residue distance map or a contact map. Instead, the shade of green indicates the expected distance error in Ångströms (Å), ranging from 0 Å to an arbitrary cut-off of 31 Å. The colour at (x, y) corresponds to the expected distance error in the residue x’s position when the predicted and the true structures are aligned on residue y.

A dark green tile corresponds to a good prediction (low error), whereas a light green tile indicates poor prediction (high error). For example, when aligning on residue 300:

  • We’re confident in the relative position of residue 200
  • We’re not confident in the relative position of residue 600

The two low-error, dark green squares correspond to the two domains. By clicking and dragging, you can highlight these squares on the structure. If you want to remove the highlighting, click the cross icon.

When selecting an off-diagonal region, the plot visually represents the relationship between the selected ranges on the sequence and structure. The x range corresponds to the selection for scored residues, highlighted in orange, while the y range of aligned residues is highlighted in emerald green.

The high PAE values across the whole inter-domain region indicate that for this particular protein, AlphaFold does not reliably predict the relative position of the domains.

Let’s consider another inter-domain example, the human protein encoded by DIP2B (Q9P265).

In this case, we have confidence in the relative position of scored residues around 1450 when aligned with residues around 850, suggesting a packing between the small central domains.

Note that the PAE scores are asymmetrical, meaning there might be variations in PAE values between (x,y) and (y,x) positions. This is particularly relevant for loop regions with highly uncertain orientations, as seen on the DNA topoisomerase 3 (Q8T2T7).

 


Last updated

Last updated in AlphaFold DB version 2022-11-01, created with the AlphaFold Monomer v2.0 pipeline.

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Jumper, J et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021).
Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024).
If you use data from AlphaMissense in your work, please cite the following paper:
Cheng, J et al. Accurate proteome-wide missense variant effect prediction with AlphaMissense. Science (2023).

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