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Transcription factor

Unreviewed
Reference proteome

AF-G5EGB2-F1-v4

DownloadPDB file mmCIF file Predicted aligned error

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Information

Structure viewer
Model Confidence
pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test (lDDT-Cα). It is a measure of local accuracy - for interpreting larger scale features like relative domain positions see the “predicted aligned error” plot and corresponding tutorial. Confidence bands are used to colour-code the residues in the 3D viewer. The exact pLDDT value is shown when you mouseover the structure or the sequence. It can also be found in the B-factor fields of the downloadable coordinate files.

 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.

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SequenceNo structure available
Structure Tools
  • 9:35:09
    Mol* Plugin 4.5.0 [8/22/2024, 2:35:37 PM]
Model Confidence
pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test (lDDT-Cα). It is a measure of local accuracy - for interpreting larger scale features like relative domain positions see the “predicted aligned error” plot and corresponding tutorial. Confidence bands are used to colour-code the residues in the 3D viewer. The exact pLDDT value is shown when you mouseover the structure or the sequence. It can also be found in the B-factor fields of the downloadable coordinate files.
 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.
Scored residueAligned residue
01002003004000100200300400
  • 0
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
Expected position error (Ångströms)

Predicted aligned error (PAE)

Click and drag a box on the PAE viewer to select regions of the structure and highlight them on the 3D viewer.

PAE data is useful for assessing inter-domain accuracy – go to Help section below for more information.

Similar structures Discover similar structures from the Protein Data Bank (PDB) and the AlphaFold Database clustered to 50% sequence identity (AFDB50) using Foldseek.
PDB ID
and chain
DescriptionSpecies Residue
range
E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
Seq.
identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
Res.
(Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
Align
in 3D
Align in 3D
PDB ID and
chain
2i13_A_3
Description

Zinc finger and SCAN domain-containing protein 2

Zinc finger and SCAN domain...

Zinc finger and SCAN domain-containing protein 2
Species

Mus musculus

Mus musculus

Residue range

218 - 388Q07230

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.90e-12
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
39.2%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
1.96
Align in 3D
PDB ID and
chain
5v3g_A_3
Description

C2H2-type domain-containing protein

C2H2-type domain-containing...

C2H2-type domain-containing protein
Species

Homo sapiens

Homo sapiens

Residue range

301 - 470D9IWL3

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
9.17e-12
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
37.1%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.42
Align in 3D
PDB ID and
chain
8sss_D_1
Description

Transcriptional repressor CTCF

Transcriptional repressor C...

Transcriptional repressor CTCF
Species

Homo sapiens

Homo sapiens

Residue range

263 - 465P49711

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.05e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
27.2%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.30
Align in 3D
PDB ID and
chain
5v3g_D_3
Description

C2H2-type domain-containing protein

C2H2-type domain-containing...

C2H2-type domain-containing protein
Species

Homo sapiens

Homo sapiens

Residue range

301 - 470D9IWL3

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.19e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
36.4%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.42
Align in 3D
PDB ID and
chain
8sst_A_1
Description

Transcriptional repressor CTCF

Transcriptional repressor C...

Transcriptional repressor CTCF
Species

Homo sapiens

Homo sapiens

Residue range

263 - 465P49711

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.65e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
28.6%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.19
Align in 3D
PDB ID and
chain
2i13_B_3
Description

Zinc finger and SCAN domain-containing protein 2

Zinc finger and SCAN domain...

Zinc finger and SCAN domain-containing protein 2
Species

Mus musculus

Mus musculus

Residue range

218 - 388Q07230

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
2.29e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
43%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
1.96
Align in 3D
PDB ID and
chain
6ml6_A_1
Description

Zinc finger and BTB domain-containing protein 24

Zinc finger and BTB domain-...

Zinc finger and BTB domain-containing protein 24
Species

Mus musculus

Mus musculus

Residue range

375 - 519Q80X44

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
2.79e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
36.7%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
1.54
Align in 3D
PDB ID and
chain
6ml4_A_1
Description

Zinc finger and BTB domain-containing protein 24

Zinc finger and BTB domain-...

Zinc finger and BTB domain-containing protein 24
Species

Mus musculus

Mus musculus

Residue range

375 - 519Q80X44

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
2.98e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
36.7%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
1.48
Align in 3D
PDB ID and
chain
8sss_A_1
Description

Transcriptional repressor CTCF

Transcriptional repressor C...

Transcriptional repressor CTCF
Species

Homo sapiens

Homo sapiens

Residue range

263 - 465P49711

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
4.14e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
31.6%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.30
Align in 3D
PDB ID and
chain
5t0u_D_1
Description

Transcriptional repressor CTCF

Transcriptional repressor C...

Transcriptional repressor CTCF
Species

Homo sapiens

Homo sapiens

Residue range

294 - 465P49711

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
4.42e-11
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
29.5%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
3.20
Items per page:
1 – 10 of 180
AF-G5EGB2-F1
Current entry

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Aligned in 3D
Rotate and zoom structures in the 3D
viewer. Structures are aligned using alpha
carbon atoms as reference points. Once
aligned, RMSD values will appear in the
list below; lower values indicate greater
similarity between the two structures.
Toggle structures in both PDB and AFDB tables to view and align them with the entry structure in the 3D viewer. You can also download the aligned coordinates of the toggled structures and the entry structure.
Structure similarity clusterPredicted structures in the AlphaFold Protein Structure Database clustered using MMseqs2 and Foldseek. This data is provided by the AFDB Clusters.

AlphaFold database protein sequences clustered by the MMseqs2 algorithm (Steinegger M. and Soeding J., Nat. Commun. 9, 2018). Each cluster is comprised of sequences that fulfil two criteria: maintaining a maximum sequence identity of 50% and achieving a 90% bi-directional sequence overlap with the longest sequence of the cluster representative.

AFDB accession DescriptionSpecies
Sequence length
Average pLDDT
AFDB accessionAF-A0A5N6KJN1-F1
Unreviewed
Reference proteome
Description C2H2-type domain-containing protein
C2H2-type domain-containing protein
SpeciesMonilinia laxa
Monilinia laxa
Sequence length 427 Average pLDDT 56.47
AFDB accessionAF-A0A6P4KS37-F1
Unreviewed
Reference proteome
Description zinc finger protein 320
zinc finger protein 320
SpeciesDrosophila bipectinata
Drosophila bipectinata
Sequence length 431 Average pLDDT 55.69
AFDB accessionAF-A0A6P5UKK7-F1
Unreviewed
Reference proteome
Description zinc finger protein 84
zinc finger protein 84
SpeciesDrosophila obscura
Drosophila obscura
Sequence length 442 Average pLDDT 55.69
AFDB accessionAF-A0A6I8UMJ6-F1
Unreviewed
Reference proteome
Description zinc finger protein 28
zinc finger protein 28
SpeciesDrosophila pseudoobscura pseudoobscura

Drosophila pseudoobscura pseudoobscura...

Drosophila pseudoobscura pseudoobscura
Sequence length 442 Average pLDDT 55.41
AFDB accessionAF-A0A6P7T363-F1
Unreviewed
Reference proteome
Description zinc finger protein 184-like
zinc finger protein 184-like
SpeciesOctopus vulgaris
Octopus vulgaris
Sequence length 435 Average pLDDT 54.72
AFDB accessionAF-A0A6J2XX53-F1
Unreviewed
Reference proteome
Description zinc finger protein 484-like isoform X1

zinc finger protein 484-like isoform X1 ...

zinc finger protein 484-like isoform X1
SpeciesSitophilus oryzae
Sitophilus oryzae
Sequence length 477 Average pLDDT 53.94
AFDB accessionAF-A0A0M4F656-F1
Unreviewed
Reference proteome
Description CG42726
CG42726
SpeciesDrosophila busckii
Drosophila busckii
Sequence length 419 Average pLDDT 52.72
AFDB accessionAF-A0A199VD53-F1
Unreviewed
Reference proteome
Description Zinc finger protein ZAT4
Zinc finger protein ZAT4
SpeciesAnanas comosus
Ananas comosus
Sequence length 442 Average pLDDT 52.62
AFDB accessionAF-A0A6V7QX22-F1
Unreviewed
Description Uncharacterized protein
Uncharacterized protein
SpeciesAnanas comosus var. bracteatus
Ananas comosus var. bracteatus
Sequence length 522 Average pLDDT 51.75
AFDB accessionAF-A0A6J2XVW3-F1
Unreviewed
Reference proteome
Description putative zinc finger protein 840 isoform X3

putative zinc finger protein 840 isoform X3 ...

putative zinc finger protein 840 isoform X3
SpeciesSitophilus oryzae
Sitophilus oryzae
Sequence length 410 Average pLDDT 50.12
Items per page:
1 – 10 of 21

Help

Visit our online training course

How to interpret the Predicted Aligned Error

 

The Predicted Aligned Error (PAE) measures the confidence in the relative position of two residues within the predicted structure, providing insight into the reliability of relative position and orientations of different domains. Consider the human protein encoded by the gene GNE (Q9Y223).

GNE has two distinct domains according to experimentally determined structures in the Protein Data Bank (PDBe-KB). Does AlphaFold confidently predict their relative positions? We can use the interactive Predicted Aligned Error (PAE) plot to answer this question.

The PAE plot is not an inter-residue distance map or a contact map. Instead, the shade of green indicates the expected distance error in Ångströms (Å), ranging from 0 Å to an arbitrary cut-off of 31 Å. The colour at (x, y) corresponds to the expected distance error in the residue x’s position when the predicted and the true structures are aligned on residue y.

A dark green tile corresponds to a good prediction (low error), whereas a light green tile indicates poor prediction (high error). For example, when aligning on residue 300:

  • We’re confident in the relative position of residue 200
  • We’re not confident in the relative position of residue 600

The two low-error, dark green squares correspond to the two domains. By clicking and dragging, you can highlight these squares on the structure. If you want to remove the highlighting, click the cross icon.

When selecting an off-diagonal region, the plot visually represents the relationship between the selected ranges on the sequence and structure. The x range corresponds to the selection for scored residues, highlighted in orange, while the y range of aligned residues is highlighted in emerald green.

The high PAE values across the whole inter-domain region indicate that for this particular protein, AlphaFold does not reliably predict the relative position of the domains.

Let’s consider another inter-domain example, the human protein encoded by DIP2B (Q9P265).

In this case, we have confidence in the relative position of scored residues around 1450 when aligned with residues around 850, suggesting a packing between the small central domains.

Note that the PAE scores are asymmetrical, meaning there might be variations in PAE values between (x,y) and (y,x) positions. This is particularly relevant for loop regions with highly uncertain orientations, as seen on the DNA topoisomerase 3 (Q8T2T7).

 


Last updated

Last updated in AlphaFold DB version 2022-11-01, created with the AlphaFold Monomer v2.0 pipeline.

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If you make use of an AlphaFold prediction, please cite the following papers:
Jumper, J et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021).
Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024).
If you use data from AlphaMissense in your work, please cite the following paper:
Cheng, J et al. Accurate proteome-wide missense variant effect prediction with AlphaMissense. Science (2023).

AlphaFold Data Copyright (2022) DeepMind Technologies Limited.
AlphaMissense Copyright (2023) DeepMind Technologies Limited.

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