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CCN family member 2

Reviewed
Reference proteome

AF-P29279-F1-v4

DownloadPDB file mmCIF file Predicted aligned error

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Information

Structure viewer
 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.

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SequenceNo structure available
Structure Tools
  • 16:02:27
    Mol* Plugin 4.5.0 [8/22/2024, 2:35:37 PM]
 Very high (pLDDT > 90)
 High (90 > pLDDT > 70)
 Low (70 > pLDDT > 50)
 Very low (pLDDT < 50)
AlphaFold produces a per-residue model confidence score (pLDDT) between 0 and 100. Some regions below 50 pLDDT may be unstructured in isolation.

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Scored residueAligned residue
050100150200250300050100150200250300
  • 0
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
Expected position error (Ångströms)

Predicted aligned error (PAE)

Click and drag a box on the PAE viewer to select regions of the structure and highlight them on the 3D viewer.

PAE data is useful for assessing inter-domain accuracy – go to Help section below for more information.

The heatmap is unavailable for mobile devices. Please use a larger screen to see the heatmap.

134950100150200250300100200300
50100150200250300GAVLISTCMDNEQRKHFYWPGAVLISTCMDNEQRKHFYWPAlternative amino acidsResidue sequence number
BenignUncertainPathogenic Reference
Amino acid present in the reference UniProt protein sequence, based on HG38
  • 0.0
  • 0.2
  • 0.3
  • 0.4
  • 0.5
  • 0.6
  • 0.7
  • 0.8
  • 1.0
  • -
Filter by category
0
0.34
0.00.34
0.34
0.56
0.340.564
0.56
1
0.5641
Similar structures Discover similar structures from the Protein Data Bank (PDB) and the AlphaFold Database clustered to 50% sequence identity (AFDB50) using Foldseek.
PDB ID
and chain
DescriptionSpecies Residue
range
E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
Seq.
identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
Res.
(Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
Align
in 3D
Align in 3D
PDB ID and
chain
5nb8_C_1
Description

CCN family member 3

CCN family member 3

Species

Rattus norvegicus

Rattus norvegicus

Residue range

100 - 195Q9QZQ5

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.50e-7
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
53.6%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.10
Align in 3D
PDB ID and
chain
5nb8_B_1
Description

CCN family member 3

CCN family member 3

Species

Rattus norvegicus

Rattus norvegicus

Residue range

100 - 195Q9QZQ5

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.58e-7
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
53.6%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.10
Align in 3D
PDB ID and
chain
5nb8_A_1
Description

CCN family member 3

CCN family member 3

Species

Rattus norvegicus

Rattus norvegicus

Residue range

100 - 195Q9QZQ5

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.85e-7
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
53.6%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.10
Align in 3D
PDB ID and
chain
5nb8_D_1
Description

CCN family member 3

CCN family member 3

Species

Rattus norvegicus

Rattus norvegicus

Residue range

100 - 195Q9QZQ5

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.96e-7
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
53.6%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
2.10
Align in 3D
PDB ID and
chain
6rk1_B_1
Description

CCN family member 3

CCN family member 3

Species

Rattus norvegicus

Rattus norvegicus

Residue range

195 - 249Q9QZQ5

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.14e-4
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
76%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
1.63
Align in 3D
PDB ID and
chain
6rk1_A_1
Description

CCN family member 3

CCN family member 3

Species

Rattus norvegicus

Rattus norvegicus

Residue range

195 - 249Q9QZQ5

E-value
Likelihood of a match between
the query and target sequence
in a structural alignment.
The lower the E-value, the more
significant the alignment.
1.48e-4
Seq. identity
Percentage of identical
residues between aligned
sequences over the
aligned length.
76%
Resolution (Å)
Resolution. Indicates the level
of detail present in the 3D
structure. Smaller value means
finer details of the structure
and higher quality.
1.63
Items per page:
1 – 6 of 6
AF-P29279-F1
Current entry

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Aligned in 3D
Rotate and zoom structures in the 3D
viewer. Structures are aligned using alpha
carbon atoms as reference points. Once
aligned, RMSD values will appear in the
list below; lower values indicate greater
similarity between the two structures.
Toggle structures in both PDB and AFDB tables to view and align them with the entry structure in the 3D viewer. You can also download the aligned coordinates of the toggled structures and the entry structure.
Structure similarity clusterPredicted structures in the AlphaFold Protein Structure Database clustered using MMseqs2 and Foldseek. This data is provided by the AFDB Clusters.

AlphaFold database protein sequences clustered by the MMseqs2 algorithm (Steinegger M. and Soeding J., Nat. Commun. 9, 2018). Each cluster is comprised of sequences that fulfil two criteria: maintaining a maximum sequence identity of 50% and achieving a 90% bi-directional sequence overlap with the longest sequence of the cluster representative.

AFDB accession DescriptionSpecies
Sequence length
Average pLDDT
AFDB accessionAF-G7MQD1-F1
Unreviewed
Reference proteome
Description Uncharacterized protein
Uncharacterized protein
SpeciesMacaca mulatta
Macaca mulatta
Sequence length 284 Average pLDDT 79.31
AFDB accessionAF-D2HL25-F1
Unreviewed
Description Uncharacterized protein
Uncharacterized protein
SpeciesAiluropoda melanoleuca
Ailuropoda melanoleuca
Sequence length 294 Average pLDDT 79.12
AFDB accessionAF-Q5RI33-F1
Unreviewed
Reference proteome
Description Cellular communication network factor 2a

Cellular communication network factor 2a ...

Cellular communication network factor 2a
SpeciesDanio rerio
Danio rerio
Sequence length 345 Average pLDDT 79.02
AFDB accessionAF-O19113-F1
Reviewed (Swiss-Prot)
Reference proteome
Description CCN family member 2
CCN family member 2
SpeciesSus scrofa
Sus scrofa
Sequence length 349 Average pLDDT 78.1
AFDB accessionAF-O18739-F1
Reviewed (Swiss-Prot)
Reference proteome
Description CCN family member 2
CCN family member 2
SpeciesBos taurus
Bos taurus
Sequence length 349 Average pLDDT 78.02
AFDB accessionAF-A0A0B3AIQ6-F1
Unreviewed
Reference proteome
Description EF hand domain/PKD protein
EF hand domain/PKD protein
SpeciesArchaeon GW2011_AR11
Archaeon GW2011_AR11
Sequence length 375 Average pLDDT 77.81
AFDB accessionAF-A0A2K5J333-F1
Unreviewed
Reference proteome
Description Cellular communication network factor 2

Cellular communication network factor 2 ...

Cellular communication network factor 2
SpeciesColobus angolensis palliatus
Colobus angolensis palliatus
Sequence length 301 Average pLDDT 77.69
AFDB accessionAF-Q9QZQ5-F1
Reviewed (Swiss-Prot)
Reference proteome
Description CCN family member 3
CCN family member 3
SpeciesRattus norvegicus
Rattus norvegicus
Sequence length 351 Average pLDDT 77.64
AFDB accessionAF-P29279-F1
Reviewed (Swiss-Prot)
Reference proteome
Description CCN family member 2
CCN family member 2
SpeciesHomo sapiens
Homo sapiens
Sequence length 349 Average pLDDT 77.64
AFDB accessionAF-A0A2K6U3G0-F1
Unreviewed
Reference proteome
Description Cellular communication network factor 2

Cellular communication network factor 2 ...

Cellular communication network factor 2
SpeciesSaimiri boliviensis boliviensis
Saimiri boliviensis boliviensis
Sequence length 297 Average pLDDT 77.5
Items per page:
1 – 10 of 1507

Help

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How to interpret the Predicted Aligned Error

 

The Predicted Aligned Error (PAE) measures the confidence in the relative position of two residues within the predicted structure, providing insight into the reliability of relative position and orientations of different domains. Consider the human protein encoded by the gene GNE (Q9Y223).

GNE has two distinct domains according to experimentally determined structures in the Protein Data Bank (PDBe-KB). Does AlphaFold confidently predict their relative positions? We can use the interactive Predicted Aligned Error (PAE) plot to answer this question.

The PAE plot is not an inter-residue distance map or a contact map. Instead, the shade of green indicates the expected distance error in Ångströms (Å), ranging from 0 Å to an arbitrary cut-off of 31 Å. The colour at (x, y) corresponds to the expected distance error in the residue x’s position when the predicted and the true structures are aligned on residue y.

A dark green tile corresponds to a good prediction (low error), whereas a light green tile indicates poor prediction (high error). For example, when aligning on residue 300:

  • We’re confident in the relative position of residue 200
  • We’re not confident in the relative position of residue 600

The two low-error, dark green squares correspond to the two domains. By clicking and dragging, you can highlight these squares on the structure. If you want to remove the highlighting, click the cross icon.

When selecting an off-diagonal region, the plot visually represents the relationship between the selected ranges on the sequence and structure. The x range corresponds to the selection for scored residues, highlighted in orange, while the y range of aligned residues is highlighted in emerald green.

The high PAE values across the whole inter-domain region indicate that for this particular protein, AlphaFold does not reliably predict the relative position of the domains.

Let’s consider another inter-domain example, the human protein encoded by DIP2B (Q9P265).

In this case, we have confidence in the relative position of scored residues around 1450 when aligned with residues around 850, suggesting a packing between the small central domains.

Note that the PAE scores are asymmetrical, meaning there might be variations in PAE values between (x,y) and (y,x) positions. This is particularly relevant for loop regions with highly uncertain orientations, as seen on the DNA topoisomerase 3 (Q8T2T7).

 


Last updated

Last updated in AlphaFold DB version 2022-11-01, created with the AlphaFold Monomer v2.0 pipeline.

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If you make use of an AlphaFold prediction, please cite the following papers:
Jumper, J et al. Highly accurate protein structure prediction with AlphaFold. Nature (2021).
Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024).
If you use data from AlphaMissense in your work, please cite the following paper:
Cheng, J et al. Accurate proteome-wide missense variant effect prediction with AlphaMissense. Science (2023).

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AlphaMissense Copyright (2023) DeepMind Technologies Limited.

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